과제정보
연구 과제 주관 기관 : 한양대학교
생물정신의학 (Korean Journal of Biological Psychiatry)
- 제3권2호
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- Pages.181-190
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- 1996
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- 1225-8709(pISSN)
- /
- 2005-7571(eISSN)
전기경련충격시 경쟁적 역전사 중합효소연쇄반응(CRT-PCR)을 이용한 흰쥐 뇌 c-fos 유전자의 발현 양식 분석
Quantitative RT-PCR for Measuring C-fos Gene Expression in Rat Brain after ECS
-
양병환
(한양대학교 의과대학 정신과학교실 및 정신건강연구소)
;
- 이제욱 (한양대학교 의과대학 정신과학교실) ;
- 박응철 (한양대학교 의과대학 정신과학교실) ;
- 유재학 ;
- 조광원 (한양대학교 이과대학 생화학과) ;
- 양보기 (한양대학교 이과대학 생화학과) ;
-
채영규
(한양대학교 이과대학 생화학과)
-
Yang, Byung-Hwan
(Department of Neuropsychiatry, School of Medicine & The Mental Health Research Institute, Hanyang University)
;
- Lee, Jei-Wook (Department of Neuropsychiatry, School of Medicine, Hanyang University) ;
- Park, Eung-Chul (Department of Neuropsychiatry, School of Medicine, Hanyang University) ;
- Yu, Jae-Hak (Department of Neuropsychiatry, Stony Brook Hospital) ;
- Cho, Goang-Won (Department of Biochimistry College of Science, Hanyang University) ;
- Yang, Bo-Gee (Department of Biochimistry College of Science, Hanyang University) ;
-
Chai, Young-Gyu
(Department of Biochimistry College of Science, Hanyang University)
- 발행 : 1996.12.25
초록
To clarify the mechanism of action of electroconvulsive shack(ECS) in respect to molecular biology, and to detect the quantitative amount of change of c-fos gene expression after ECS in the rat's brain, the authors obtained brain specimens from the striatum, cerebral cortex, hippocampus, and cerebellum. Each brain was removed within 30min. after ECS(130V, 0.5sec) and ECS-sham. Then we performed RT-PCR. The results are 1) ECS was found to affect the expression of immediate early genes. 2) the cerebral cortex and hippocampus was more influenced by ECS thon in the cerebellum and striatum. From these results, we can suggest that ECS is related to the mechanism of cognition, mood, memory which is correlated to cerebral cortex and hippocampus.