BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
권호 표지

Purification and Characterization of Phytoferritin

  • Oh, Suk-Heung (Department of Biotechnology, Woosuk University) ;
  • Cho, Sung-Woo (Department of Biochemistry, College of Medicine, University of Ulsan) ;
  • Kwon, Tae-Ho (Institute for Molecular Biology and Genetics, and Faculty of Biological Sciences, Chonbuk National University) ;
  • Yang, Moon-Sik (Institute for Molecular Biology and Genetics, and Faculty of Biological Sciences, Chonbuk National University)
  • 투고 : 1996.08.08
  • 발행 : 1996.11.30
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초록

Ferritins from germinated pumpkin seeds were isolated by ammonium sulfate precipitation (0.55 saturation), ion-exchange chromatography on DEAE-cellulose, and gel filtration chromatographies on Sephacryl S-300 and Sephadex G-100. Pumpkin ferritin contains less iron than soybean ferritin. Pumpkin ferritin cross-reacted with anti-soybean ferritin antiserum made in rabbit, and showed two distinct antibody reactive bands, both of equal intensity. The pumpkin ferritins corresponding to the two bands were separable by centrifugation in a sucrose gradient (20~50%). The molecular weights of the native pumpkin ferritins based on the estimation of sucrose gradient centrifugation, gel filtration on Sephacryl S-300 and non-denaturing polyacrylamide gel electrophoresis appeared to be: 530~580 KD (the large molecular weight pumpkin ferritin) and 330-360 KD (the small molecular weight pumpkin ferritin) The large molecular weight pumpkin ferritin contains less iron. Both pumpkin ferritins cross-reacted with anti-soybean ferritin antibody with a spur formation suggesting partial antigenic recognition.

키워드

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