부착단백질이 사람 치수세포의 부착 및 증식에 미치는 영향에 관한 연구

THE EFFECT OF ADHESIVE GLYCOPROTEINS ON THE ATTACHMENT AND PROLIFERATION OF HUMAN PULPAL CELLS

The purpose of this vitro study was to evaluate attachment and proliferation of human pulpal cells to the attachment glycoprotein-coated and non-coated culture dishes. Well known adhesive glycoproteins were used, such as type I collagen, type IV collagen, fibronectin, laminin, and vitronection. Each adhesive glycoproteins applied onto the culture dishes. In this study, the protein coated and non-coated dishes were classified as each groups. Human pulpal cells onto each culture dishes. After 90 minute, 4 hour and 24 hour incubation attached cells in each group were counted with hematocytometer for evaluation of the attachemnt of human pulpal cells. The configurations of attached human pulpal cells were done by SEM observation. The results as follows : 1. After 90 minute incubation the score of attachment of human pulpal cells was best in laminin-coated group among groups. Then fibronectin, type IV collagen group were better, and all proteins were higher than control. 2. After 4 hour incubation the numbers of attachment of human pulpal cells were most in fibronectin coated group. 3. After 24 hour incubation all of adhesive glycoproteins showed high and similar attachemtn effect to human pulpal cells. 4. In SEM observation, fibronectin and type IV collagen groups showed well spreaded human pulpal cells, then laminin group was moderately spreaded, and vitronectin group was mildly spreaded as well as control group.