Korean Journal of Veterinary Research (대한수의학회지)
- Volume 36 Issue 4
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- Pages.845-858
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- 1996
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Immunocytochemistry, In situ hybridization and electron microscopy for early diagnosis of Aujeszky's in living pigs
오제스키병의 생체 조기진단을 위한 면역세포화학, In situ hybridization 및 전자현미경적 연구
- Moon, Oun-kyong (National Veterinary Research Institute) ;
- Kim, Soon-bok (College of Veterinary Medicine, Gyeongsang National University) ;
- Sur, Jung-hyang (Detp. of Veterinary and Biomedical Sciences, University of Nebraska) ;
- Song, Geun-suk (College of Veterinary Medicine, Gyeongsang National University) ;
- Nho, Whan-gook (Pusan Veterinary Service Laboratory)
- Received : 1996.03.13
- Published : 1996.12.25
Abstract
The purpose of this study was to establish early diagnostic methods for the detection of Aujeszky's disease viral antigens and nucleic acid in nasal cells, and buffy coats from experimentally infected living pigs by a combination of immunocytochemistry, in situ hybridization with digoxigenin(DIG)-labled probe and electron microscopy. Forty days old piglets were inoculated intranasally with
Keywords
- immunocytochemistry;
- digoxigenin-labeled in situ hybridization;
- electron microscopy;
- Aujeszky's disease virus;
- nasal cells;
- buffy coats