Korean Journal of Veterinary Research (대한수의학회지)
- Volume 36 Issue 2
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- Pages.379-387
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- 1996
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Isolation of Escherichia coli O157:H7 from animal feces and biochemical characteristics of Verotoxin-2 produced by these strains II. Purification and characterization of Verotoxin-2 Produced by Escherichia coli O157:H7 Isolated from animal feces
동물분변에서 Escherichia coli O157:H7의 분리 및 이들 균이 생산하는 Verotoxin-2의 생물화학적 특성 II. 동물분변에서 분리한 Escherichia coli O157:H7으로부터 Verotoxin-2의 정제 및 특성
- Cha, In-ho (Pusan Institute of Health and Environment) ;
- Kim, Yong-hwan (College of Veterinary Kyeongsang National University)
- Received : 1995.12.15
- Published : 1996.06.25
Abstract
The objects of the present study were to establish the method of purification, subunit dissociation of verotoxin-2 (VT2) produced by Escherichia coli O157:H7, and to investigate the characteristics of purified verotoxin-2 such as molecular weight and composition of amino acid. The results were summerized as follows; Verotoxin-2 was extracted by addition of polymyxin B sulfate into bacterial cell lysate prepared from Escherichia coli O157:H7(KSC109). As an initial step, the bacterial cell lysate was precipitated with 30% saturated ammonium sulfate. The precipitated crude toxin was then subjected to anion-exchange, chromatofocusing and cation-exchange chromatography. Using this scheme, we obtained highly purified toxin with a specific activity of