Korean Journal of Veterinary Research (대한수의학회지)
- Volume 36 Issue 2
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- Pages.349-358
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- 1996
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Immunohistochemical detection of antigens in tissue sections of experimentally infected hamsters
Leptospira canicola 실험적 감염햄스타의 조직절편내 면역조직화학적 균체항원검출
- Ku, Bok-gyeong (Veterinary Research Institute, Anyang RDA) ;
- Kim, Soon-bok (College of Veterinary Medicine Gyeongsang National University) ;
- Sur, Jung-hang (College of Veterinary Medicine Gyeongsang National University) ;
- Moon, Oun-kyong (Veterinary Research Institute, Anyang RDA) ;
- Jung, Suk-chan (Veterinary Research Institute, Anyang RDA) ;
- Park, Jung-moon (Korea Microbiology Lab. LTD,) ;
- Park, Yong-ho (College of Veterinary Medicine Seoul National University) ;
- Mun, Jin-san (Veterinary Research Institute, Anyang RDA)
- 구복경 (수의과학연구소) ;
- 김순복 (경상대학교 수의과대학) ;
- 서정향 (경상대학교 수의과대학) ;
- 문윤경 (수의과학연구소) ;
- 정석찬 (수의과학연구소) ;
- 박정문 (한국미생물연구소) ;
- 박용호 (서울대학교 수의과대학) ;
- 문진산 (수의과학연구소)
- Received : 1995.11.24
- Published : 1996.06.25
Abstract
The present study was done to detect Leptospira canicola antigens in paraffin sections from experimentally infected hamsters with PAP method. The anti-Leptospira canicola serum used as first antibody was prepared by immunizing rabbits with Leptospira canicola. Positive reactions were detected in interstitium, tubular epithelial cells and glomerulus of the kidney, and in the hepatic sinusoid. With PAP method, leptospiral antigens were detected at the 48 hours after bacterial infection, but isolation, silver stain, and dark field microscopic examination of the samples did 48 hours later than PAP method. The results suggested that PAP method is considered as a reliable tool for confirmative diagnosis of this disease.