Korean Journal of Veterinary Research (대한수의학회지)
- Volume 36 Issue 2
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- Pages.305-312
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- 1996
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- 2466-1384(pISSN)
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- 2466-1392(eISSN)
Ionic currents elicited by the hypotonic solution in hamster eggs
저장성 용액에 노출된 햄스터 난자에 관찰되는 이온전류의 변화
- Choi, Won-yeong (College of Veterinary Medicine and Department of Physiology) ;
- Kim, Yang-mi (College of Medicine, Gyeongsang National University) ;
- Haan, Jae-hee (College of Medicine, Gyeongsang National University) ;
- Huh, Il-oh (College of Medicine, Gyeongsang National University) ;
- Park, Choon-ok (College of Medicine, Gyeongsang National University) ;
- Hong, Seong-geun (College of Medicine, Gyeongsang National University) ;
- Pyu, Pan-dong (College of Veterinary Medicine Seoul National University) ;
- Kim, Jong-shu (College of Veterinary Medicine and Department of Physiology)
- 최원영 (경상대학교 수의과대학 약리학교실) ;
- 김양미 (경상대학교 의과대학 생리학교실) ;
- 한재희 (경상대학교 의과대학 생리학교실) ;
- 허일오 (경상대학교 의과대학 생리학교실) ;
- 박춘옥 (경상대학교 의과대학 생리학교실) ;
- 홍성근 (경상대학교 의과대학 생리학교실) ;
- 류판동 (서울대학교 수의과대학) ;
- 김종수 (경상대학교 수의과대학 약리학교실)
- Received : 1995.12.04
- Published : 1996.06.25
Abstract
Cell volume regulatory mechanisms are usually disclosed by exposure of cell to anisotonic media. If a cell is suddenly exposed to hypotonic media, it swells initially like an osmometer but within minutes regains its original cell volume. This behavior has been labelled as regulatory cell volume decrease(RVD). RVD is believed to result from the loss of permeable ions through the membrane. In this study, we examined hypotonically induced changes in the membrance currents involved in RVD by using whole cell voltage clamp technique in the unfertilized hamster egg. At -40mV of the holding potential, the stationary current was maintained in the hamster egg exposed to isotonic solution composed of, mainly, 115mM NaCl and 40mM mannitol. Hypotonic solution was prepared by removing mannitol. Therefore, the concentrations of
Keywords
- Cell volume regulatory mechanism;
- hypotonic media;
- $Cl^{-}$ efflux regulatory cell volume decrease(RVD)