한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)
- 제29권5호
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- Pages.637-642
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- 1996
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- 0374-8111(pISSN)
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- 2287-8815(eISSN)
해양 미생물 Pseudomonas sp.의 유전자 재조합 Alginate Lyase의 특성
Characteristics of Recombinant Alginate Lyase of a Marine Bacterium, Pseudomonas sp.
- 김영옥 (부경대학교 생물공학과) ;
- 김구택 (부경대학교 생물공학과) ;
- 김현국 (부경대학교 생물공학과) ;
- 김대경 (부경대학교 생물공학과) ;
- 허성회 (부경대학교 해양학과) ;
- 공인수 (부경대학교 생물공학과)
- KIM Young-Ok (Department of Biotechnology & Bioengineering, Pukyong National University) ;
- KIM Gu-Taek (Department of Biotechnology & Bioengineering, Pukyong National University) ;
- KIM Hyun-Kuk (Department of Biotechnology & Bioengineering, Pukyong National University) ;
- KIM Dae-Kyung (Department of Biotechnology & Bioengineering, Pukyong National University) ;
- HUH Sung-Hoi (Department of Oceanography, Pukyong National University) ;
- KONG In-Soo (Department of Biotechnology & Bioengineering, Pukyong National University)
- 발행 : 1996.09.01
초록
해양으로부터 alginate lyase분해능이 있는 Pseudomonas sp.을 분리하여 이 균으로부터 alginate lyase 유전자를 E. coli에 cloning 하여 재조합 alginate lyase의 특성을 검토하였다. 재조합 alginate lyase는 E. coli에서
We isolated a marine bacterium, Pseudomonas sp,, which could produce the enzyme of alginate lyase, and cloned the alginate lyase gene in Escherichia coli. The cloned DNA was overexpressed with approximately