Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)
- Volume 29 Issue 5
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- Pages.637-642
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- 1996
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- 0374-8111(pISSN)
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- 2287-8815(eISSN)
Characteristics of Recombinant Alginate Lyase of a Marine Bacterium, Pseudomonas sp.
해양 미생물 Pseudomonas sp.의 유전자 재조합 Alginate Lyase의 특성
- KIM Young-Ok (Department of Biotechnology & Bioengineering, Pukyong National University) ;
- KIM Gu-Taek (Department of Biotechnology & Bioengineering, Pukyong National University) ;
- KIM Hyun-Kuk (Department of Biotechnology & Bioengineering, Pukyong National University) ;
- KIM Dae-Kyung (Department of Biotechnology & Bioengineering, Pukyong National University) ;
- HUH Sung-Hoi (Department of Oceanography, Pukyong National University) ;
- KONG In-Soo (Department of Biotechnology & Bioengineering, Pukyong National University)
- 김영옥 (부경대학교 생물공학과) ;
- 김구택 (부경대학교 생물공학과) ;
- 김현국 (부경대학교 생물공학과) ;
- 김대경 (부경대학교 생물공학과) ;
- 허성회 (부경대학교 해양학과) ;
- 공인수 (부경대학교 생물공학과)
- Published : 1996.09.01
Abstract
We isolated a marine bacterium, Pseudomonas sp,, which could produce the enzyme of alginate lyase, and cloned the alginate lyase gene in Escherichia coli. The cloned DNA was overexpressed with approximately
해양으로부터 alginate lyase분해능이 있는 Pseudomonas sp.을 분리하여 이 균으로부터 alginate lyase 유전자를 E. coli에 cloning 하여 재조합 alginate lyase의 특성을 검토하였다. 재조합 alginate lyase는 E. coli에서