한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)
- 제29권6호
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- Pages.761-769
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- 1996
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- 0374-8111(pISSN)
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- 2287-8815(eISSN)
Random Amplified Polymorphic DNA (RAPD) Identification of Genetic Variation in Chlorella species
- CHO Jung Jong (Department of Microbiology, Pukyong National University) ;
- KIM Yong-Tae (Department of Chemistry, Aoyama Gakuin University) ;
- HUR Sung Bum (Department of Aquaculture, Pukyong National University) ;
- KIM Young Tae (Department of Microbiology, Pukyong National University)
- 발행 : 1996.11.01
초록
The random amplified polymorphic DNA (RAPD) technique was used to characterize 18 reference strains of microalgae, mostly Chlorella species, collected from various localities around Korea peninsular. Eighteen strains consist of four genera of the family marine Chlorella from 12 samples, two genera of fresh water Chlorella from three samples, and three genera on Nannochloris. Twenty 10-mer anonymous primers were screened for amplification of genomic DNA extracted from samples using the CTAB extraction method. Nineteen of these oligonucleotide primers were positive or band producing. Three of 20 random primers (OPA 10, OPA 12, and OPA 18) resulted in both clear band and a high degree of reproducibility and showed some potential to be used to discriminate individual samples of both genetically hetero-and homogeneous populations, in determining phylogenetic relationships between species within a genus and developing individual fingerprints for each samples.