Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
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IN VITRO STEM CELL SUPPRESSION OF MACROPHAGE INFLAMMATORY $PROTEIN-1{\alpha}$

Macrophage Inflammatory $Protein-1{\alpha}$의 조혈간세포(造血幹細胞) 억제 작용에 관한 실험적 연구

  • Suh, Ki-Hang (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Chonbuk National University) ;
  • Ko, Seung-O (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Chonbuk National University) ;
  • Shin, Hyo-Keun (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Chonbuk National University) ;
  • Kim, Oh-Whan (Dept. of Oral & Maxillofacial Surgery, College of Dentistry, Chonbuk National University)
  • 서기항 (전북대학교 치의학과 구강악안면외과학교실) ;
  • 고승오 (전북대학교 치의학과 구강악안면외과학교실) ;
  • 신효근 (전북대학교 치의학과 구강악안면외과학교실) ;
  • 김오환 (전북대학교 치의학과 구강악안면외과학교실)
  • Published : 1996.06.30
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Abstract

The proliferation of bone marrow stem cell compartment is thought to be under both positive and negative controls by cytokines and colony stimulation factors. Macrophage inflammatory $protein-1{\alpha}(MIP-1{\alpha})$ has been assessed for its potential to protect hematopoietic stem cells from cytotoxic effects of a cycle-specific antineoplastic agents. We have tested the ability of $MIP-1{\alpha}$ to suppress the proliferation of stem cell line Du.528.101 in variety of active status by using $[^{3}H]-thymidine$ incorporation test. The results were as follows. 1. The effect of $MIP-1{\alpha}$ on steady-state Du.528.101 cell represented the cell growth suppression at the concentration of 10, 50, 100nM of $MIP-1{\alpha}$(P<0.001). 2. $MIP-1{\alpha}$ stimulated the proliferation of Du.528.101 cells previously treated with IL-1 at the concentration of 5, 50nM of $MIP-1{\alpha}$(P<0.01). 3. The suppression effect of MIP-1 on Du.528.101 cells at the concentration of 5, 50nM was shown when cells were treated with $MIP-1{\alpha}$ before activation with $IL-1{\beta}(P<0.01)$. 4. The growth rate of synchronized cells were slower than that of non-synchronized ones, and $MIP-1{\alpha}$ represented the similar suppression effect on both synchronized and non-synchronized cells.

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