한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제24권1호
  • /
  • Pages.19-26
  • /
  • 1996
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

방선균 분리주가 생산하는 Phospholipase C 저해물질인 MT-2617-2B의 분리 및 특성

Isolation and Characterization of MT2617-2B, a Phospholipase C Inhibitor Produced by an Actinomycetes Isolate

  • 고학룡 (한국과학기술연구원, 생명공학연구소, 미생물화학연구그룹) ;
  • 이현선 (한국과학기술연구원, 생명공학연구소, 미생물화학연구그룹) ;
  • 오원근 (한국과학기술연구원, 생명공학연구소, 미생물화학연구그룹) ;
  • 안순철 (한국과학기술연구원, 생명공학연구소, 미생물화학연구그룹) ;
  • 김보연 (한국과학기술연구원, 생명공학연구소, 미생물화학연구그룹) ;
  • 강대욱 (한국과학기술연구원, 생명공학연구소, 미생물화학연구그룹) ;
  • 민태익 (한국과학기술연구원, 생명공학연구소, 미생물화학연구그룹) ;
  • 안종석 (한국과학기술연구원, 생명공학연구소, 미생물화학연구그룹)
  • Ko, Hack-Ryong (Microbial Chemistry Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Lee, Hyun-Sun (Microbial Chemistry Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Oh, Won-Keun (Microbial Chemistry Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Ahn, Soon-Cheol (Microbial Chemistry Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Kim, Bo-Yeon (Microbial Chemistry Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Kang, Dae-Ook (Microbial Chemistry Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Mheen, Tae-Ick (Microbial Chemistry Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Ahn, Jong-Seog (Microbial Chemistry Research Group, Korea Research Institute of Bioscience and Biotechnology, KIST)
  • 발행 : 1996.02.01
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초록

A phospholipase C (PLC) inhibitor (MT267-2B) was isolated from the culture broth of actinomycetes isolate MT2617-2 by the extraction with n-butanol and column chromatographic techniques. The molecular weight of the inhibitor was 1057, by the spectroscopic analyses of IR, $^{13}C$-and $^{1}H$-NMR and ESI-MS. The chemical structure of MT2617-2B was found to be a macrolide compound consisted of a hemiketal ring, polyhydroxyl and polymethyl groups, which had a malonate and guanidine group as its side chain. MT2617-2B produced its two isomers having the same molecular weight by standing in methanol solution at room temperature. Therefore, MT2617-2B was identified as copiamycin and niphithricin A, macrolide antibiotics. The values of $IC_{50}$ against PLC-${\gamma}$1 and PLC-${\beta}$1 were 25 and 50${\mu}$g/ml, respectively. MT2617-2B had antimicrobial activities against Staphylococcus aureus and Candida albicans, but not against Escherichia coli.

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