YAKHAK HOEJI (약학회지)
- Volume 40 Issue 1
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- Pages.59-64
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- 1996
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
Transformation of Gram-Positive Bacteria by Electroporation
Electroporation을 이용한 그람 양성 세균의 형질전환
- Oh, Tae-Gwon (College of Pharmacy, Seuol National University) ;
- Kim, Byung-Kak (College of Pharmacy, Seuol National University) ;
- Choi, Eung-Chil (College of Pharmacy, Seuol National University)
- Published : 1996.02.01
Abstract
Gram-positive bacteria, Bacillus subtilis BR151 and Staphylococcus aureus RN4220 were transformed with high efficiency by electroporation. The cells were incubated until late log phase, washed three times with 10% glycerol, 1mM HEPES, 12% sucrose and resuspended to