약학회지 (YAKHAK HOEJI)
- 제40권1호
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- Pages.59-64
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- 1996
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- 0377-9556(pISSN)
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- 2383-9457(eISSN)
Electroporation을 이용한 그람 양성 세균의 형질전환
Transformation of Gram-Positive Bacteria by Electroporation
- Oh, Tae-Gwon (College of Pharmacy, Seuol National University) ;
- Kim, Byung-Kak (College of Pharmacy, Seuol National University) ;
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Choi, Eung-Chil
(College of Pharmacy, Seuol National University)
- 발행 : 1996.02.01
초록
Gram-positive bacteria, Bacillus subtilis BR151 and Staphylococcus aureus RN4220 were transformed with high efficiency by electroporation. The cells were incubated until late log phase, washed three times with 10% glycerol, 1mM HEPES, 12% sucrose and resuspended to