Induction of ${\beta}$-Glucosidase and ${\alpha}$-Rhammosidase of Bacteroides JY-6 by Flavonoid Glycosides

플라보노이드배당체에 의한 Bacteroides JY-6의 ${\beta}$-글루코시다제 및 ${\alpha}$-람노시다제의 유도

Optimal medium for growth and glycosidases production of Bacteroides JY-6, an human intestinal bacterium, was general anaerobic medium or tryptic soy broth containing sod ium thioglycolate and ascorbic acid. By cocultivation of Staphylococcus R-48, Bacteroides JY-6 could be cultured in LB broth unable to culture JY-6. Heated Staphylococcus R-48 was also the inducer of the production of Bacteroides JY-6 glycosidases. These glycosidases were induced well by natural flavonoid glycosides, such as poncirin, naringin and rutin, but were not by synthetic substrates, p-nitrophenyl ${\beta$-D-glucopyranoside and p-nitrophenyl ${\alpha}$-L-rhanmopyranoside.