YAKHAK HOEJI (약학회지)
- Volume 40 Issue 2
- /
- Pages.212-217
- /
- 1996
- /
- 0377-9556(pISSN)
- /
- 2383-9457(eISSN)
Improvement of Carbapenem Antibiotics Productivity in S. cattleya by Transformation
형질전환에 의한 S. cattleya의 카바페넴 항생제 생산성 향상
- Park, Ji-Sun (College of Pharmacy, Ewha Womans University) ;
- Lee, Kang-Man (College of Pharmacy, Ewha Womans University)
- Published : 1996.04.01
Abstract
Streptomyces cattleya is a producer of carbapenem antibiotics, thienamycin and N-acetylthienamycin, which have potent and broad-spectrum antibacterial activities. We stud ied on strain improvement for antibiotic productivity of S. cattleya by transformation technique which employed S.cattleya protoplasts and chromosomal DNAs of glutamic acid producers: Corynebacterium glutamicum and Arthrobacter simplex. 150 Transformant strains were cultured and bioassayed using Bacillus subtilis and Staphylococcus aureus as test organisms. 8.7% of transformants tested showed 1.4~2.6 fold higher productivities than wild type which produced