한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제24권1호
  • /
  • Pages.101-104
  • /
  • 1996
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

방선균주 7489가 생산하는 DNA Topoisomerase I 저해제에 관한 연구

DNA Toposiomerase I Inhibitor by Streptomyces sp. 7489

  • 이동선 (경북대학교 미생물학과) ;
  • 하상철 (한국과학기술연구원 생명공학연구소) ;
  • 이상용 (경북대학교 미생물학과) ;
  • 김종국 (경북대학교 미생물학과) ;
  • 홍순덕 (경북대학교 미생물학과)
  • Lee, Dong-Sun (Department of Microbiology, College of Natural Science, Kyungpook National University) ;
  • Ha, Sang-Chul (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Lee, Sang-Yong (Department of Microbiology, College of Natural Science, Kyungpook National University) ;
  • Kim, Jong-Guk (Department of Microbiology, College of Natural Science, Kyungpook National University) ;
  • Hong, Soon-Duck (Department of Microbiology, College of Natural Science, Kyungpook National University)
  • 발행 : 1996.02.01
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초록

During the screening of inhibitor of DNA topoisomerase I from microbial secondary metabolites, Streptomyces melanosporofaciens 7489 which was capable of producing high level of inhibitor was selected from soil. The active compound (7489-1) was purified from the culture broth by solvent extraction, silica gel column chromatography and HPLC. The inhibitor was identified as dibutyl phthalate by spectroscopic methods of UV, $^{1}H$-NMR, $^{13}C$-NMR, DEPT and EI-MS. 7489-1 showed a strong inhibitory activity against topoisomerase I with 10 ${\mu}$M of $IC_{50}$ value.

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