한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제24권1호
  • /
  • Pages.82-86
  • /
  • 1996
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Streptomyces tubercidicus ME-9189 균주가 생산하는 nucleoside계 제초 활성 물질

A Herbicidal Nucleoside Compound isolated from Streptomyces tubercidicus ME-9189

  • 김원곤 (한국과학기술원 생명공학연구소) ;
  • 김종평 (한국과학기술원 생명공학연구소) ;
  • 김창진 (한국과학기술원 생명공학연구소) ;
  • 유익동 (한국과학기술원 생명공학연구소)
  • Kim, Won-Gon (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Kim, Jong-Pyung (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Kim, Chang-Jin (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Yoo, Ick-Dong (Korea Research Institute of Bioscience and Biotechnology, KIST)
  • 발행 : 1996.02.01
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초록

Three thousand microbial strains collected from different sources were screened for herbicidal activity. A strain of ME-9189 showed herbicidal activity against Digitaria sanguinalis and Portulaca oleracea was isolated from a mountainy soil. Based on taxonomic studies, the strain was identified as Streptomyces tubercidicus. The active compound of ME-9189 was purified from the culture broth by charcoal, silica gel, sephadex LH-20 column chromatography and crystalization, consecutively. The ME-9189 compound was identified as tubercidin by spectroscopic methods of UV, $^{1}H$ and $^{13}C$-NMR, and EIMS. In the bioassay, growth of radish shoot and root was inhibited by 50% with tubercidin treatment of 10 ppm, showing 2 times higher activity than that of herbicidin A and similar to that of toyocamycin.

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