Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 24 Issue 1
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- Pages.72-76
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- 1996
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Expression of Escherichia coli ${\beta}$ -galactosidase Gene by New Transfer Vector of Baculovirus
새로운 Baculovirus 전이벡터를 이용한 Escherichia coli ${\beta}$ -galactosidase 유전자의 발현
- Woo, Soo-Dong (Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul Naitonal University) ;
- Kim, Woo-Jin (Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul Naitonal University) ;
- Kim, Hye-Seong (Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul Naitonal University) ;
- Jin, Byung-Rae (Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul Naitonal University) ;
- Kang, Seok-Kwon (Department of Agricultural Biology, College of Agriculture and Life Sciences, Seoul Naitonal University)
- 우수동 (서울대학교 농업생명과학대학 농생물학과) ;
- 김우진 (서울대학교 농업생명과학대학 농생물학과) ;
- 김혜성 (서울대학교 농업생명과학대학 농생물학과) ;
- 진병래 (서울대학교 농업생명과학대학 농생물학과) ;
- 강석권 (서울대학교 농업생명과학대학 농생물학과)
- Published : 1996.02.01
Abstract
To investigate the expression efficiency of new transfer vector of Bombyx mori nuclear polyhedrosis virus (BmNPV), Escherichia coli lacZ gene was inserted into new transfer vector pBmKSK1, under the control of polyhedrin promoter and expressed in BmN-4 cells and larvae of silkworm, Bombyx mori. The recombinant virus containing lacZ gene was isolated from BmN-4 cells coinfected with transfer vectro pBmKSK1-LacZ and wild type BmNPV genome, and analysed by Southern blotting. The expression of