Expression of an Active Adenylate Forming Domain of Peptide Synthetase

Peptide Synthetase의 활성 Adenylate 형성 Domain의 발현

  • 김연옥 (원광대학교 자연과학대학 화학과) ;
  • 김기영 (원광대학교 의과대학 병리학실) ;
  • 이성 (한서대학교 식품 생물공학과) ;
  • 이영행 (원광대학교 자연과학대학 화학과) ;
  • 유병수 (원광대학교 자연과학대학 화학과)
  • Published : 1996.02.01

Abstract

The plasmid pK8 was constructed to verify the existence of an adenylate domain in peptide synthetase by using pGC12. 1.2 kb fragment, coding tyrocidine synthetase 1 (123 kDa) was deleted, and 79.6 kDa one was expressed in Escherichia coli XL1-blue. The truncated multienzyme activated phenylalanine and substrate analogues with comparable kinetics as the over expressed synthetase. ATP-[$^{32}P$]PPi exchange reaction was measured for the enzyme assay.

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