The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 26 Issue 1
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- Pages.115-120
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- 1996
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- 1225-2344(pISSN)
Establishment of Transgenic Mouse with the E6 and E7 Genes of Human Papillomavirus Type 16
인간 Papillomavirus의 E6, E7 유전자를 이용한 Transgenic Mouse의 확립
- Hwang, Yong-Il (Department of Food Engineering, Kyungnam University) ;
- Lee, Seung-Cheol (Department of Food Engineering, Kyungnam University) ;
- Kim, Hyun-Su (R&D Center of Cheil Foods & Chemicals, Co.)
- Published : 1996.06.30
Abstract
Human papillomavirus (HPV), especially type 16 and 18, has been closely associated with carcinomas and uterine cevical cancer, recently. From in vitro assays, E6 and E7 genes of HPV16 are closely linked with transformation of cell lines of rodent fibroplasts. However, the transforming activity of E6 and E7 genes of HPV type 16 in vivo has not been fully elucidated. For explaining this mechanism, we prepared a expression system with the promoter of mouse mammary tumorvirus long terminal repeat and E6E7's open reading frames. This expression system was introduced in rodent cell lines, No. 7, 3Y1 and shown normal transforming abilities. And, we produced transgenic mice with E6, E7 expression system. These transgenic mice were confirmed from Southern blot analysis. One male of them was observed enlargement of the testis after 5 months postdelivery.
Keywords
- Human papillomavirus;
- E6 and E7 genes;
- mouse mammary tumorvirus long terminal repeat;
- transgenic mice;
- E6, E7 expression system