The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 26 Issue 2
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- Pages.251-258
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- 1996
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- 1225-2344(pISSN)
Sequence and Phylogenetic Analysis of V3 Region of Human Immunodeficiency Virus Type 1 Strains Isolated from Korean Patients
한국인 인면역결핍 바이러스의 V3 Loop 염기서열 분석 및 계통발생학적 분석
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Kim, Young-Bong
(Department of Life Science, University of Sogang) ;
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Cho, Young-Keol
(Department of Microbiology, College of Medicine, University of Ulsan) ;
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Lee, Hee-Jung
(Department of Microbiology, College of Medicine, University of Ulsan) ;
- Chung, Koo-Hun (Chong Kun Dang Research Institute) ;
- Kim, Jung-Woo (Chong Kun Dang Research Institute) ;
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Kim, Yoo-Kyum
(Department of Microbiology, College of Medicine, University of Ulsan) ;
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Yang, Jai-Myung
(Department of Life Science, University of Sogang)
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김영봉
(서강대학교 생명과학과) ;
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조영걸
(울산의대 미생물학교실) ;
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이희정
(울산의대 미생물학교실) ;
- 정구헌 (종근당 종합연구소) ;
- 김정우 (종근당 종합연구소) ;
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김유겸
(울산의대 미생물학교실) ;
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양재명
(서강대학교 생명과학과)
- Published : 1996.12.30
Abstract
The V3 loop, a hypervariable domain of envelope glycoprotein, has an essential role in viral infectivity and has a major epitope for type-specific neutralizing antibody. In order to investigate genetic diversity of V3 region of gp120 of human immunodeficiency virus type 1 (HIV-1) isolated from Korean patients, DNA sequences encoding the C2 to V3 region were amplified by nested polymerase chain reaction (PCR) from uncultured peripheral blood mononuclear cells obtained from 15 HIV-1 seropositive patients and nucleotide sequences were determined. All nucleotide sequences from fifteen patients were compared with 8 distinctive subtypes (A-H) and another subtype O. Phylogenetic analysis was carried out with PHYLIP ver 3.5 (Dnapars) program. Of the 15 isolates, 14 HIV-1 subjects were clustered with subtype B, while one was clustered with subtype C. Intra-subtype B distance at the nucleotide and deduced amino acid level were maximum 17.7% and 37.0%, respectively. Intra-patient distance at the nucleotide and deduced amino acid level were maximum 7.3% and 17.8%, respectively. Analysis of the nucleotide sequences revealed that Korean types have relatively well conserved sequences. These findings could be useful for assessing the source of infection and developing an AIDS vaccine.