대한바이러스학회지 (The Journal of Korean Society of Virology)

대한바이러스학회 (The Korean Society of Virology)
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일본뇌염 바이러스 Nakayama-NIH주와 국내에서 분리된 일본 뇌염 바이러스주의 유전적 차이 및 항원성 차이의 조사

Antigenic and Genetic Differences between the Prototype Nakayama-NIH Strain and Korean Strains of Japanese Encephalitis Virus

  • 조해월 (국립보건원 바이러스질환부 신경계 바이러스과) ;
  • 남재환 (국립보건원 바이러스질환부 신경계 바이러스과) ;
  • 이유진 (국립보건원 바이러스질환부 신경계 바이러스과) ;
  • 김은정 (국립보건원 바이러스질환부 신경계 바이러스과) ;
  • 이호동 (국립보건원 바이러스질환부 신경계 바이러스과) ;
  • 윤경식 (국립보건원 바이러스질환부 신경계 바이러스과) ;
  • 고현철 (한양대학교 의과대학 약리학교실)
  • Cho, Hae-Wol (Division of Arboviruses, Department of Virology, National Institute of Health) ;
  • Nam, Jae-Hwan (Division of Arboviruses, Department of Virology, National Institute of Health) ;
  • Lee, Yoo-Jin (Division of Arboviruses, Department of Virology, National Institute of Health) ;
  • Kim, Eung-Jung (Division of Arboviruses, Department of Virology, National Institute of Health) ;
  • Lee, Ho-Dong (Division of Arboviruses, Department of Virology, National Institute of Health) ;
  • Yun, Gyeong-Sik (Division of Arboviruses, Department of Virology, National Institute of Health) ;
  • Koh, Hyun-Chul (Department of pharmacology, College of medicine, Hanyang University)
  • 발행 : 1996.12.30
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초록

The characterization of the 5 Korean isolates (K96P10, K94P05, K91P55, K87P39, and K82P01) of Japanese encephalitis virus (JEV) was compared with JE virus prototype Nakayama-NIH (NKY-NIH) using prM/M and envelope gene sequences of the JEV genome and phylogenetic analysis. The antigenic analysis of these viruses were done by the cross-hamagglutination inhibition (HI) test using polyclonal antibodies against Korean isolates and NKY-NIH. The sequence homology of the Korean isolates and NKY-NIH ranged between 87.4 % - 95.6 % at the nucleotide level and between 98.2 % - 97.2 % at the amino acid level over the E nucleotides compared. Alignment of E protein amino acid sequences revealed that residue positions E89, E129, E221, E244, E327, E366, E459, and E477 characterized the Korean strains. According to phylogenetic analysis bases on the E nucleotide, there are at least 2 genetic types of JEV existing in Korea and Korean strains were distinct from NKY-NIH. However, the cross HI test results of all the Korean isolates were serologically no different from NKY-NIH strain.

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