The Journal of Korean Society of Virology (대한바이러스학회지)
- Volume 26 Issue 2
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- Pages.191-204
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- 1996
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- 1225-2344(pISSN)
Antigenic and Genetic Differences between the Prototype Nakayama-NIH Strain and Korean Strains of Japanese Encephalitis Virus
일본뇌염 바이러스 Nakayama-NIH주와 국내에서 분리된 일본 뇌염 바이러스주의 유전적 차이 및 항원성 차이의 조사
- Cho, Hae-Wol (Division of Arboviruses, Department of Virology, National Institute of Health) ;
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Nam, Jae-Hwan
(Division of Arboviruses, Department of Virology, National Institute of Health) ;
- Lee, Yoo-Jin (Division of Arboviruses, Department of Virology, National Institute of Health) ;
- Kim, Eung-Jung (Division of Arboviruses, Department of Virology, National Institute of Health) ;
- Lee, Ho-Dong (Division of Arboviruses, Department of Virology, National Institute of Health) ;
- Yun, Gyeong-Sik (Division of Arboviruses, Department of Virology, National Institute of Health) ;
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Koh, Hyun-Chul
(Department of pharmacology, College of medicine, Hanyang University)
- 조해월 (국립보건원 바이러스질환부 신경계 바이러스과) ;
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남재환
(국립보건원 바이러스질환부 신경계 바이러스과) ;
- 이유진 (국립보건원 바이러스질환부 신경계 바이러스과) ;
- 김은정 (국립보건원 바이러스질환부 신경계 바이러스과) ;
- 이호동 (국립보건원 바이러스질환부 신경계 바이러스과) ;
- 윤경식 (국립보건원 바이러스질환부 신경계 바이러스과) ;
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고현철
(한양대학교 의과대학 약리학교실)
- Published : 1996.12.30
Abstract
The characterization of the 5 Korean isolates (K96P10, K94P05, K91P55, K87P39, and K82P01) of Japanese encephalitis virus (JEV) was compared with JE virus prototype Nakayama-NIH (NKY-NIH) using prM/M and envelope gene sequences of the JEV genome and phylogenetic analysis. The antigenic analysis of these viruses were done by the cross-hamagglutination inhibition (HI) test using polyclonal antibodies against Korean isolates and NKY-NIH. The sequence homology of the Korean isolates and NKY-NIH ranged between 87.4 % - 95.6 % at the nucleotide level and between 98.2 % - 97.2 % at the amino acid level over the E nucleotides compared. Alignment of E protein amino acid sequences revealed that residue positions E89, E129, E221, E244, E327, E366, E459, and E477 characterized the Korean strains. According to phylogenetic analysis bases on the E nucleotide, there are at least 2 genetic types of JEV existing in Korea and Korean strains were distinct from NKY-NIH. However, the cross HI test results of all the Korean isolates were serologically no different from NKY-NIH strain.
Keywords
- Japanese Encephalitis virus;
- prM and envelope gene;
- Cross-hamagglutination inhibition;
- Phylogenetic analysis