Adenovirus types in pediatric gastroenteritis in seoul

서울 지역 장염환아에서 분리되는 아데노바이러스 형별

  • Cho, Eun-Kyung (Department of Microbiology, Hanyang Univ. Coll. of Medicine) ;
  • Lee, Kyu-Man (Department of Clinical Pathology, Hallym Univ. College of Medicine) ;
  • Chung, Yong-Hoon (Department of Microbiology, Hanyang Univ. Coll. of Medicine) ;
  • Cho, Yang-Ja (Department of Microbiology, Hanyang Univ. Coll. of Medicine) ;
  • Kim, Kyung-Hee (Department of Microbiology, Hanyang Univ. Coll. of Medicine)
  • 조은경 (한양대학교 의과대학 미생물학교실) ;
  • 이규만 (한림대학교 임상병리학교실) ;
  • 정용훈 (한양대학교 의과대학 미생물학교실) ;
  • 조양자 (한양대학교 의과대학 미생물학교실) ;
  • 김경희 (한양대학교 의과대학 미생물학교실)
  • Published : 1996.06.02

Abstract

Adenoviruses(Ad) are considered to be second only to rotaviruses as the most significant cause of gastroenteritis in young children in Korea and thus it is essential to know the full spectrum of Ad serotypes routinely present in stool specimens from symptomatic patients. Sixty-six Ad isolates and three questionable ones collected over a 2-year peiord were typed by standard microneutralization, restriction endonuclease digestion and PCR of viral DNA to be able to evaluate these assays comprehensively for their ability to identify Ad associated with gastroenteritis. A total of sixty-one isolates(88.4%) were typed: the predominant types were Ad type 41(Ad41)(26.2%), Ad2(19.7%), Ad40(14.8%), Ad5(9.8%), and Ad7(9.8%) which together accounted for almost 80% of the isolates. The remaining virus isolates were typed as Ad1, 31, 34, 3, 25 and a mixture of 40/41. The incidence of Ad31(4.9%) or Ad3(1.6%) was relatively insignificant. DNA restriction analysis(77.5%) proved to be better than serum neutralization but not so when compared to a PCR-based assay for identification of the enteric Ad serotypes(90%) in stool specimens. In this work, the PCR-based assay was evaluated as a tool for the rapid, yet highly sensitive identification of adenoviral DNA sequences in fresh clinical stool specimens.

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Acknowledgement

Supported by : 한양대학교