Pediatric Infection and Vaccine
- Volume 3 Issue 2
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- Pages.133-138
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- 1996
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- 2384-1079(pISSN)
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- 2384-1087(eISSN)
Detection of Methicillin-Resistant Staphylococcus aureus by In Vitro Enzymatic Amplification of MecA and FemA Gene
메티실린 내성 황색 포도상 구균에서 mecA, femA 유전자의 임상적 의의
- Park, Jung-Eun (Department of Pediatrics, Sung-Ae General Hospital) ;
- Kim, Taek-Sun (Department of Pediatrics, Sung-Ae General Hospital) ;
- Park, Su-Sung (Department of Pediatrics, Sung-Ae General Hospital) ;
- Kim, Eun-Ryoung (Department of Pediatrics, Sung-Ae General Hospital) ;
- Kim, Il-Su (Institute of Genetic Engeering research, Sung-Ae General Hospital) ;
- Ann, Il-Young (Institute of Genetic Engeering research, Sung-Ae General Hospital) ;
- Kim, Young-Jin (Institute of Genetic Engeering research, Sung-Ae General Hospital) ;
- Kim, Jae-Jong (Bioneeer Corp. R&D Center) ;
- Kang, Sung-Ok (Bioneeer Corp. R&D Center) ;
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Park, Han-Ho
(Bioneeer Corp. R&D Center)
- 박정은 (성애병원 소아과) ;
- 김택선 (성애병원 소아과) ;
- 박수성 (성애병원 소아과) ;
- 김은령 (성애병원 소아과) ;
- 김일수 (성애 유전공학 연구소) ;
- 안일영 (성애 유전공학 연구소) ;
- 김영진 (성애 유전공학 연구소) ;
- 김재종 (바이오니아 부설 연구소) ;
- 강성옥 (바이오니아 부설 연구소) ;
-
박한오
(바이오니아 부설 연구소)
- Published : 1996.11.20
Abstract
Purpose : In the treatment of MRSA infection, rapid detection of MRSA is extremely important. The mecA gene codes the new drug resistant polypeptides called PBP2' which mediates the clinically relevant resistance to all beta-lactam antibiotics. The identical mecA gene has been found in coagulase-negative staphylococcus with the methicillin-resistant phenotype. On the other hand, the femA gene was absent from coagulase negative staphylococcus strains with the methicillin resistant phenotype. This study is aimed at early detection and definite diagnosis of MRSA. Methods : A total of 24 MRSA strains were studied. All strains were tested for antimicrobial susceptibility and purified DNA. We amplified both mecA and femA genes by PCR in 24 strains. Results : In MRSA all the 16 strains (100%) carried femA gene and 11 strains (68.7%) carried mecA gene. In contrast, in methicillin sensitive staphylococcus all the 8 strains (100%) carried femA and only 3 strains (37.5%) were detected mecA. Conclusions : As results, there are difference in the phenotype and genotype of methicillin resistance by PCR of mecA and femA. Such disparities between methicillin resistance and the presence of mecA gene suggest the presence of control gene of the mecA.