Journal of Microbiology and Biotechnology
- Volume 6 Issue 6
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- Pages.402-406
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- 1996
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Purification and Characterization of Inulin Fructotransferase (Depolymerizing) from Arthrobacter sp. A-6
- PARK, JEONG-BOK (Graduate School of Biotechnology, Korea University) ;
- YONG-JIN CHOI (Graduate School of Biotechnology, Korea University)
- Published : 1996.12.01
Abstract
Inulin fructotransferase (depolymerizing) (EC 2.4.1.93) was purified 34-fold from the culture broth of Arthrobacter sp. A-6 by using a combination of ammonium sulfate fractionation, DEAE-Sepharose CL-6B chromatography and Sephacryl S-200 gel filtration. The purified enzyme converts inulin into di-D-fructofuranose dianhydride III(DFA III) and small quantities of fructo-oligosaccharides. The temperature and pH optima of the enzyme were
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