Journal of Microbiology and Biotechnology
- Volume 6 Issue 6
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- Pages.445-450
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- 1996
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Pyridoxatin, an Inhibitor of Gelatinase A with Cytotoxic Activity
- Lee, Ho-Jae (Enzyme Inhibition Research Unit, Biomolecule Research Division) ;
- Chung, Myung-Chul (Enzyme Inhibition Research Unit, Biomolecule Research Division Korea Research Institute of Bioscience and Biotechnology(KRIBB), KIST) ;
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Lee, Choong-Hwan
(Enzyme Inhibition Research Unit, Biomolecule Research Division Korea Research Institute of Bioscience and Biotechnology(KRIBB), KIST) ;
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Chun, Hyo-Kon
(Enzyme Inhibition Research Unit, Biomolecule Research Division Korea Research Institute of Bioscience and Biotechnology(KRIBB), KIST) ;
- Kim, Hwan-Mook (Bioresources Program, Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB), KIST) ;
- Kho, Yung-Hee (Enzyme Inhibition Research Unit, Biomolecule Research Division Korea Research Institute of Bioscience and Biotechnology(KRIBB), KIST)
- Published : 1996.12.01
Abstract
Gelatinase A is a member of the matrix metalloproteinases that play an important role in cancer invasion and metastasis. In the course of screening gelatinase A inhibitors from microbial sources, a fungal strain PT-262 showed a strong inhibitory activity. The strain was identified as Chaunopycnis alba on the basis of its morphological characteristics. The inhibitor was isolated from acetone extract of mycelial cake by sequential chromatographies on MCI-gel, Sephadex LH-20, and a reverse-phase HPLC column. The purified inhibitor was identified as pyridoxatin by its physico-chemical properties and spectroscopic analysis. Pyridoxatin is not a peptide analog and has cyclic hydroxamic acid moiety. It inhibited activated gelatinase A with an
Keywords
- pyridoxatin;
- gelatinase A inhibitor;
- cytotoxicity;
- DNA synthesis inhibitor;
- Chaunopycnis alba PT-262