Journal of Microbiology and Biotechnology
- Volume 6 Issue 6
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- Pages.375-380
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- 1996
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Cloning and Characterization of GL-7-ACA Acylase Gene from Pseudomonas sp. GK16
- LEE, YOUNG-SIK (Graduate School of Biotechnology, Korea University) ;
- HAN-CHUL YANG (Graduate School of Biotechnology, Korea University) ;
- SUNG-SOO PARK (Graduate School of Biotechnology, Korea University)
- Published : 1996.12.01
Abstract
The gene coding for glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase was cloned from Pseudomonas sp. GK16 and some of its characteristics were analyzed. The complete nucleotide sequence revealed that the putative open reading frame is 2160 bases long and encodes 720 amino acids. By SDS-PAGE three proteins, approximately corresponding to 70, 54 and 16 kDa of molecular weight, were detected in E. coli cells carrying pGAP18. The largest protein should be a precursor which is not processed yet, while the other two proteins must be derived from the precursor by the proteolytic processing.
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