Enzymatic Conversion of Glutaryl 7-Aminocephalosporanic Acid to 7-Aminocephalosporanic Acid with an Immobilized Glutaryl 7-Aminocephalosporanic Acid Acylase

  • SHIN, HAN-JAE (Korea Research Institute of Bioscience and Biotechnology) ;
  • SEUNG-GOO LEE (Korea Research Institute of Bioscience and Biotechnology) ;
  • WANG-SIK LEE (Biotechnology Laboratory, Cong Kun Dang Corp.) ;
  • KI-HONG YOON (Department of Food Science, Woosong Sanup University)
  • Published : 1996.10.01

Abstract

Glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. SY-77-1 was immobilized with oxiran acrylic beads for the production of 7-aminocephalosporanic acid (7-ACA) from glutaryl 7-aminocephalosporanic acid (GL 7-ACA). The immobilized enzyme maintained its activity at a constant level for 7 days, but lost 30$%$ of its activity after 20 days. Optimal reaction conditions for the synthesis of 7-ACA were found to be $30^{\circ}C$ and pH 8.0 using the immobilized enzyme. For the economic production of 7-ACA, substrate and enzyme concentrations were optimized to 60 mM and 0.5 g wet weight per 10 $m\ell$ of reaction volume, respectively. Under optimized conditions, 50 mM 7-ACA was produced from 60mM GL 7-ACA within 8 h, resulting in a conversion yield of 83$%$.

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