The Effect of Glutamine on Production of Tissue-type Plasminogen Activator from Recombinant Human Melanoma Cells in Glutamine-limited Fed-batch Cultivation

  • Kim, Hyun-Goo (Division of Food and Biotechnology, Kangwon National University) ;
  • Kim, Tae-Ho (Division of Food and Biotechnology, Kangwon National University) ;
  • Kim, Dae-Seok (Division of Food and Biotechnology, Kangwon National University) ;
  • Park, Kyung-Yu (Department of Medicine, Hanllym University) ;
  • Park, jin-seo (Department of Genetic Engineering, Hanllym University) ;
  • Ahn, Chol (Division of Food and Biotechnology, Kangwon National University) ;
  • Lee, Jin-Ha (Division of Food and Biotechnology, Kangwon National University) ;
  • Lee, Hyeon-Yong (Division of Food and Biotechnology, Kangwon National University)
  • Published : 1996.08.01

Abstract

Under glutamine-limited condition, $2\times10^6$ (viable cells/ml) of maximum cell density and 13.5 ($\mu g$/ml) of tissue-type Plasminogen Activators (tPA) production were maintained by spike feeding fresh medium in fed-batch cultivation of human recombinant melanoma cells. It showed that tPA production was much seriously affected than cell growth according to initial glutamine concentrations. Above 3.4 (mmol/I) of glutamine concentration both cell growth and tPA production were not much affected by increasing initial glutamine concentration. Glutamine depleted situation was occurred at latter periods of batch and fed-batch cultivations below 5.4 (mmole/I) of initial glutamine concentration. It also showed that maximum glutamine consumption and ammonia evolution rates were closely related to initial glutamine concentrations. Maximum specific tPA production rate was estimated as $8.1\times19^{-6}$ ($\mu g$/cells/h) at 3.4(mmol/I) of glutamine concentration, which is higher than that from other batch and fed-batch processes.

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