Journal of Microbiology and Biotechnology
- 제6권3호
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- Pages.184-188
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- 1996
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Purification and Characterization of Bioemulsifier Produced by Acinetobacter sp. BE-254
- Kim, Soon-Han (Department of Microbiology, Pusan National University) ;
- Lee, Jae-Dong (Department of Microbiology, Pusan National University) ;
- Kim, Boo-Chul (Department of Microbiology, Pusan National University) ;
- Lee, Tae-Ho (Department of Microbiology, Pusan National University)
- 발행 : 1996.06.01
초록
The Acinetobacter sp. BE-254 isolated from soil sources produced a bioemulsifier in the medium supplemented with n-hexadecane. This bioemulsifier was purified by the procedures of fractionation (ammonium sulfate and chilled acetone), extraction by hexane, and column chromatography on silica gel 60. The results from various color reactions indicated that the bioemulsifier was a glycolipid. The purified emulsifier was very stable at pHs ranging from 4 to 10 and under heat treatment at