Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Cloning of Steroid $\Delta^1$-dehydrogenase Gene of Arthrobacter simplex IAM 1660

  • Bae, Moo (Department of Biological Science, Ewha Womans Uniersity) ;
  • Bae, Song-Mee (Department of Biological Science, Ewha Womans Uniersity) ;
  • Lee, Mi-Kyung (Department of Biological Science, Ewha Womans Uniersity) ;
  • Lee, Jeong-Kug (Department of Life Science, Sogang University)
  • Published : 1996.04.01
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Abstract

To clone the gene coding for steroid $\Delta^1$-dehydrogenase of Arthrobacter simplex, its genomic library was constructed with a , $\lambda$gt11 expression vector and immunoscreened with antiserum against the enzyme. One positive clone was found to carry a 1.6-kb EcoR I restriction endonuclease fragment of A. simplex DNA. The restriction map of the 1.6-kb EcoR I fragment was determined after cloning of the DNA into pBS vector.

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