Journal of Microbiology and Biotechnology
- Volume 6 Issue 2
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- Pages.98-102
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- 1996
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Production of L-DOPA by Thermostable Tyrosine Phenol-lyase of a Thermophilic Symbiobacterium Species Overexpressed in Recombinant Escherichia coli
- Lee, Seung-Goo (Microbial Conversion Resrarch Unit, Applied Microbiology Research Divistion, Korea Research Institute of Bioscience & Biotechnology, KIST) ;
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Ro, Hyeon-Su
(Microbial Conversion Resrarch Unit, Applied Microbiology Research Divistion, Korea Research Institute of Bioscience & Biotechnology, KIST) ;
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Hong, Seung-Pyo
(Microbial Conversion Resrarch Unit, Applied Microbiology Research Divistion, Korea Research Institute of Bioscience & Biotechnology, KIST) ;
- Kim, Eun-Hwa (Microbial Conversion Resrarch Unit, Applied Microbiology Research Divistion, Korea Research Institute of Bioscience & Biotechnology, KIST) ;
- Sung, Moon-Hee (Microbial Conversion Resrarch Unit, Applied Microbiology Research Divistion, Korea Research Institute of Bioscience & Biotechnology, KIST)
- Published : 1996.04.01
Abstract
A thermostable tyrosine phenol-lyase gene of a thermophilic Symbiobacterium species was cloned and overexpressed in Escherichia coli in order to produce the biocatalyst for the synthesis of 3, 4-dihy-droxyphenyl-L-alanine (L-DOPA). The substrates used for the synthetic reaction were pyrocatechol, so-dium pyruvate, and ammonium chloride. The enzyme was stable up to
Keywords
- Tyrosine phenol-lyase;
- thermostable enzyme;
- 3,4-dihy-droxyphenyl-L-alanine(L-DOPA);
- thermophilic Symbiobacterium species