Journal of Microbiology and Biotechnology
- 제6권1호
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- Pages.19-25
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- 1996
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Purification and Characteristics of Chitosanase from Bacillus sp. HW-002
- Lee , Hyean-Woo (Department of Biochmistry, Wonju College of Medicine, Yonsei University) ;
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Choi, Jong-Whan
(Department of Biochmistry, Wonju College of Medicine, Yonsei University) ;
- Han, Dong-Pyou (Department of Biochmistry, Wonju College of Medicine, Yonsei University) ;
- Park, Myoung-Jin (Department of Microbiological Engineering, College of Engineering, Konkuk University) ;
- Lee, No-Woon (Department of Microbiological Engineering, College of Engineering, Konkuk University) ;
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Yi, Dong-Heui
(Department of Microbiological Engineering, College of Engineering, Konkuk University)
- 발행 : 1996.02.01
초록
Chitosanase from Bacillus sp. HW-002 was purified with CM-cellulose column chromatography, and HPLC with DEAE- TSK gel and YMC-pack Diol 120. The purified enzyme appeared as a single band on SDS-polyacrylamide gel. The molecular weight of the enzyme was estimated to be about 46 kDa on SDS-polyacrylamide gel, and was estimated to be about 23 kDa by GFC. The optimal pH of chitosanolytic activity was about pH 5.5-6.0, and the purified enzyme was most stable at pH 5.0. The optimal temperature of chitosanolytic activity was