Journal of Microbiology and Biotechnology
- Volume 6 Issue 1
- /
- Pages.1-6
- /
- 1996
- /
- 1017-7825(pISSN)
- /
- 1738-8872(eISSN)
Purification and Characterization of an Alkaline Protease from Bacillus licheniformis NS70
- Kim, Young-Ok (Applied Microbiology Research Group, Korea Research Institute of Bioscience & Biotechnology, Korea Institute of Science and Technology) ;
- Lee, Jung-Kee (Applied Microbiology Research Group, Korea Research Institute of Bioscience & Biotechnology, Korea Institute of Science and Technology) ;
- Kim, Hyung-Kwoun (Applied Microbiology Research Group, Korea Research Institute of Bioscience & Biotechnology, Korea Institute of Science and Technology) ;
- Park, Young-Seo (Applied Microbiology Research Group, Korea Research Institute of Bioscience & Biotechnology, Korea Institute of Science and Technology) ;
- Oh, Tae-Kwang (Applied Microbiology Research Group, Korea Research Institute of Bioscience & Biotechnology, Korea Institute of Science and Technology)
- Published : 1996.02.01
Abstract
A bacterial strain NS70 producing an alkaline protease was isolated from soil samples taken near a hot spring and identified as Bacillus licheniformis by its morphological and physiological properties and cellular fatty acid analysis. The isolated alkaline protease was purified by ammonium sulfate fractionation, DEAE-, CM-, and Phenyl-Sepharose column chromatography. The molecular weight of the purified enzyme was estimated to be 32, 000 Da by sodium dodecylsulfate polyacrylamide gel electrophoresis. Its optimal pH and temperature for proteolytic activity against Hammarsten casein were 12 and