Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Dietary Capsaicin on Proto-oncogenes Expression in Various in Mice

식이 Capsaicin이 마우스의 주요 장기조직에서의 Proto-oncogenes Expression에 미치는 영향

  • Published : 1996.12.01
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Abstract

Capsaicin (8-methyl-N-vanillyl-6-nonenamide: CAP) is a mai or ingredient of hot pepper that has been used as a spicy food additive, preservative, and medicine. In this study, we evaluated the effect of dietary CAP on the selected proto-oncogene(c-jun, c-myc, H-ras, erbB, p53) expressions in various tissues of mice. Male ICR mice were divided into four groups and fed the experimental diets containing CAP at the levels of 0, 5, 20 and 100ppm for four weeks. Steady state RNA levels in various tissues were measured by slot blot hybridization assay. C-jun expression level was enhanced in stomach tissue from mice fed 20ppm CAP and significantly reduced from mice fed 100ppm CAP. The c-jun expression levels were differentially altered in organ-specific manner, Tumor suppressor gene p53 expression level appeared to be slightly increased in the liver from mice fed 20ppm CAP. These results suggested that dietary CAP differentially modulates c-jun and p53 expression in various organs.

매운맛 성분(capsaicin, CAP)이 암발생에 미치는 영향에 대한 분자적인 수준에서의 기초 정보를 확보하기 위해, 식이 CAP의 투여가 동물 조직 중 proto-oncogene 의 발현에 미치는 영향을 조사하였다. ICR mouse를 4 group으로 분류하여 각각 식이CAP 농도가 0, 5, 20, 100ppm이 되도록 조제한 먹이로 4주 동안 사육하였다. 사육기간 종료 후 동물들의 중요장기를 적출하여 total RNA를 분리하고, proto-oncogene(c-jun, c-myc, H-ras, erbB, p53)의 발현 수준을 slot blot hybridization assay를 통해 살펴 보았다. 이때, control probe로는 18SrRNA를 사용하였다. 그 결과, c-jun proto-oncogene의 발현은 각 주요 장기조직에 따라 다른 양상을 나타내었는데, 식이CAP 투여량이 증가함에 따라 간과 신장에서 그 발현이 증가하며, 위에서는 CAP 20ppm까지는 c-jun의 발현이 증가하다. 100ppm 투여시에는 감소하는 것으로 나타났으며, 비장에서는 식이CAP 투여량이 증가함에 따라 감소하는 경향을 보였다. 한편, tumor suppressor gene인 p53의 경우, 간에서만 CAP 20, 100ppm 처리시 약하게 발현되었다. 이들 결과로 보아, 식이 CAP에 의한 proto-oncogene의 발현은 CAP 투여량에 따라 그 정도를 달리하며, 그 발현 정도는 조직 특이성을 나타내는 것으로 평가된다.

Keywords

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