한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제25권4호
  • /
  • Pages.701-707
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  • 1996
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지

Aspergillus niger SFN-416 이 생산하는 Xylanase I 의 정제 및 특성

Purification and Characterization of Xylanase I from Aspergillus niger SFN-416

  • 발행 : 1996.08.01
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초록

Aspergillus niger SFN-416으로 부터 생성한 xylanase I를 분리.정제하여 특성을 조사하였다. Aspergillus niger SFN-416의 배양액을 ethanol(70%) 침전, $(NH_4)_2-SO_4$(30~90%) 침전, Sephadex G-100 chromatography 및 DEAE-Sephacel ion chromatography 등의 정제과정을 거친 결과, 10.2배 정제되었고, 정제효소의 최적 활성온도는 $50^{\circ}C$ 였다.최적 pH는 3.5이었고, pH 안정성은 6.0 이상에서 활성이 급격히 감소하였다. 또한 금속이온에 대한 효소의 활성은 대부분 억제를 보였고, 특히 $Hg^{2+}$는 18.5%로 가장 낮은 상대활성을 보였지만, $Fe^{2+}$는 117.0%, $Mn^{2+}$는 129.9%로 오히려 효소활성이 증가되었다. 정제 효소의 분자량은 SDS-PAGE에 의하여 31,000 daltons이 었으며, 유기용매에 대한 활성과 안정성은 10%의 methanol, ethanol, isopropanol 및 1-butanol 대하여 모두 낮은 활성을 나타내어 유기용매에는 안정하지 않는 것으로 생각된다.

Xylanase(EC. 3. 2. 1. 8) was purified approximately 10.2 fold from Aspergillus niger SFN-416 by a sequential process of ammonium sulfate fractionation, Sephadex G-100 gel filtration and DEAE-Sephacel ion exchange chromatography. Molecular weight of the enzyme was approximately 31,000 daltons. The optimum pH and temperature of the enzyme activity were 3.5 and $50^{\circ}C$ respectively. The enzyme activity was enhanced by $Fe^{2+}$ and $Mn^{2+}$, and inhibited by $Hg^{2+}$. The activity was decreased by addition of methanol, ethanol, isopropanol and 1-butanol at a concentration of 10%(v/v).

키워드

참고문헌

  1. Carbohydrate Research v.42 Purification, properties and mode of action of hemicellulase II by Ceratocystis paradoxa Dekker, R. F. H.;Richards, G. N.
  2. FEBS Lett. v.186 Acetyl xylan esterases in fungal cellulolytic system Biely, P.;Puls, J.;Schneider, H.
  3. Biosynthesis and biodegradation of wood components Deeker, R. F. H.
  4. FEMS Microbiol. Rev. v.104 Molecular biology of xylan degradation Thomson, J. A.
  5. Top. Enzyme Ferment. Biotechnol. v.8 Xylanase : functions, properties and application Woodward, J.
  6. Phytopathology v.57 Hemicellulose degradation in sunflower hypocotyls infected with Sclerotiorum Hancock, J. G.
  7. Phytopathology v.59 Enzymatic degradation of galactan, galactomannan and xylan by Sclerotium rolfsii Van Etten, H. D.;Bateman, D. F.
  8. Physiological Plant Pathology v.6 Polysaccharide degrading enzymes produced by Fusarium roseum 'Avenaceum' Mullen, J. M.;Bateman, D. F.
  9. ACS Symp. Ser. Viikari, L.;Kantelinen, A.;Ratto, M.;Sundgust, J.;Leatham, G. E.(ed.);Himmel, E. M.(ed.)
  10. 산업미생물학회지 v.21 β-Glucosidase를 생산하는 균주의 분리 및 조효소의 특성 박석규;문일식;성낙계
  11. J. Bacteriol. v.73 Induction of cellulase in Trichoderma viride as influenced by carbon sources and metal Mandels, M.;Reese, E. T.
  12. Anal. Chem. v.31 Use of dinitrosalicyclic acid reagent for determination of reducing sugar Miller, G. L.
  13. J. Biol. Chem. v.193 Protein estimation with the folin phenol reagent Lowry, O. H.;Resebrough, N. J.;Farr, A. L.;Randall, R. J.
  14. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T₄ Laemmli, U. K.
  15. 산업미생물학회지 v.19 Bacillus stearothermophilus가 생산하는 xylanase의 정제 및 특성 배성호;최용진
  16. Mycological Research v.98 Purification and characterization of a new xylanase from Bipolaris sorokiniana Peltonene, S.;Karjalainen, R.;Niku-Paavola, M. L.
  17. Applied and Environmental Microbiology v.59 Purification and characterization of a new xylanase(APX-II) from the fungus Aureobasidium Y-2311-1 Li, X. L.;Zhang, Z. Q.;Jeffrey, F. D. D.;Karl-Erik, L. E.;Lars, G. L.
  18. Phytopathology v.83 Purification and properties of an endoxylanase from a corn stalk rot strain of Erwinia chrysanthemi Braun, E. J.;Rodrigues, C. A.
  19. 한국생화학회지 v.25 Penicillium verruculosum으로부터 D-xylanase의 정제 및 특성 조남철;권수진;김강화;정기철
  20. Applied and Environmental Microbiology v.59 Purification and characterization of alkaline xylanase from Bacillus Polymyxa Morales, P.;Madarro, A.;Perez-Gonzalez, J. A.;Sendra, J. M.;Pinaga, J. M. F.;Flors, A.
  21. 산어미생물학회지 v.20 Bacillus sp. N-25가 생산하는 xylanase의 특성 김원곤;이찬용;이계호
  22. J. Microbiol. Biotech. v.4 Purification characterization and chemical modification of the xylanase from alkali-tolerant Bacillus sp. YA-14 Park, Y. S.;Yum, D. Y.;Hahm, B. K.;Bai, D. H.;Yu, J. H.