Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Zinc Accumulation in the Cell of Zinc-Tolerant Bacteria, Pseudomonas chlororaphis, and Recovery of Zinc from the Cells Accumulating Zinc

아연 내성균의 균체내 아연 축적특성 및 균체내 축적된 아연의 회수

  • 조주식 (경상대학교 공동실험실습관, 경상대학교 농화학과) ;
  • 한문규 (경상대학교 공동실험실습관, 경상대학교 농화학과)
  • Published : 1996.06.01
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Abstract

This study was performed to develop the biological treatment technology of wastewater polluted with heavy metals. Zinc-tolerant microorganism, such as Pseudomonas chlororaphis which possessed the ability to accumulate zinc, was isolated from industrial wastewaters polluted with various heavy metals. The characteristics of zinc accumulation in the cells, recovery of the zinc from the cells accumulating zinc, were investigated. Removal rate of zinc from the solution containing 100 mall of Zinc by zinc-tolerant microorganism was more than 90% at 48 hours after inoiulation of the microorganisms. A large number of the electron-dense granules were found mainly on thIn cell wall and membrane fractions, when determined by transmission electron microscope. Energy dispersive X- ray spectroscopy revealed that the electron-dense granules were zinc complex with the substances binding Heavy metals. The zinc accumulated into cells was not desorbed by distilled water, but more than 80% of the zinc accumulated was desorbed by 0.1M-EDTA. The residues of the cells after combustion at 55$0^{\circ}C$ amounted to about 21% of the dry weight of the cells. EDS analysis showed that the residues were comparatively pure zinc compounds containing more than 79% of zinc.

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References

  1. 한문
  2. 1989 과기처보고서 응집제 생산을 위한 생물공정기술개발(Ⅰ) 정윤철(외)
  3. 유해폐기물 관리기술 심포지움 물리화학적처리 조순행
  4. Appl. Environ. Microbiol. v.55 no.8 Effects of metals on a range of Streptomyces species Abbas,A.;C.Edwards
  5. Appl. Environ. Microbiol. v.44 Adaptation to camium by Klebsiella aerogenes growing in continuous culture proceeds mainly via formation of cadmium sulfide Aiking,H.;K.Kok;H. van Heerikhuizen;J.van't Riet
  6. Water Res. v.13 Metal removal in activitied sludge: The role of bacterial extracellular polymer Brown,M.J.;J.N.Lester
  7. Appl. Environ. Microbiol. v.40 no.2 Comparison of bacterial extracellular polymer extraction methods Brown,M.J.;J.N.Lester
  8. Water Res. v.16 Role of bacterial extracellular polymers in metal uptake in pure bacterial culture and activated sludge. Ⅱ. Effects of mean cell retention time Brown,M.J.;J.N.Lester
  9. J. Water Pollut Control Fed. v.47 Heavy metals uptake by activited sludge Cheng,M.H.;J.W.Patterson;R.A.Minear
  10. 경상대학교 박사학위논문 Characterozaton of heavy metal accumulation in the cell of heavy metal-tole-rant microorganisms and utillization of the microorganisms as heavy metal biosorbent Cho,J.S.
  11. Appl. Environ. Microbiol. v.56 no.10 Remobilization of toxic heavy metals adsorbed to bacterial wall-clay composites Flemming,C.A.;FG.Ferris;T.J.Beveridge;G.W.Bailey
  12. Biotechnol. Bioeng. v.28 Biosorption of uranium and lead by Streptomyces longwoodensis Friis,N.;P.Myers-Keith
  13. Kor. J. Environ. Agric. v.13 Utilization of microorganisms for treating wastewater polluted with heavy metals Heo,J.S.;J.S.Cho
  14. Enzyme. Microb. Technol. v.14 Leaching of heavy metals from anaerobic sewage sludge by sulfur-oxidizing bacteria Jain,D.K.;R.D.Tyagi
  15. Kor. J. Appl. Microbiol. Bioeng. v.4 no.3 Leaching of mium-tolerant bacteria and accumilation of cadmium into the bacteria cell. Kim,Y.B.;S.R.Lee
  16. Biotechnol. Bioeng. v.33 Kuyucak,N.;B.Volesky Kuyucak,N.;B.Volesky
  17. Water Res. v.13 The influence of heavy metals on a mixed bacterial population of sewage otigin in the chemostat. Lester,J.N.;R.Perry;A.H.Dado
  18. J. Gen. Microbiol. v.130 Cadmium accumulation by a Citrobacter sp. Macaskie,L.E.;A.C.R.Dean
  19. Biotechnol. Bioeng. v.28 Cadmium accumulation by immobilized cells of a Citrobacter sp. using various phosphate donors Michel,L.J.;L.E.Makaskie;A.C.R.Dean
  20. Appl. Environ. Microbiol. v.55 no.12 Baterial sorption of heavy metals Mullen,M.D.;D.C.Wolf;T.J.Beveridge;C.A.Flemming;G.W>Baily
  21. Eur. J. Appl. Microbiol. Biotechnol. v.12 Studies on the accumulation of heavy metal elements in biological systems. XVII. Selective accumulation of heavy metal ions by Chlorella regularis Nakajima,A.;T.Hirokoshi;T.Sakaguchi
  22. Appl. Microbiol. Biotechnol. v.24 Selective accumulation of heavy metals by microorganisms Nakajima,A.;T.Sakaguchi
  23. Biotechnol. Bioeng. v.26 Accumulation of heavy metal ions by Zoogloea ramigera Norberg,A.B.;H.Persson
  24. Biotechnol. Lett. v.11 no.2 The use of Zooloea ramigera in waste water treatment containing Cr(Ⅵ) and Cd(Ⅱ) ions Sag,Y.;T.Kutsal
  25. Appl. Environ. Microbiol. v.41 no.1 Microbial cell as biosorbents for heavy metals: Accumulation of uranium by Saccharomyces cerevisiae and Pseudomonas aeruginosa Stanberg,G.W.;S.E.Shumate Ⅱ;J.R.Parrott,Jr.
  26. Biotechnol. Bioeng. v.26 Ion exchange/complexation of the uranyl ion by Rhizopus bio-sorbent Treen-Sears,M.E.;B.Volesky;R.J.Neufeld
  27. Biotechnol. Bioeng. v.23 Biosorption of uranium and thorium Tsezos;B.Volesky
  28. Biotechnol. Bioeng. v.26 Recovery of uranium from biological adsorbents-desorption equilibrium Tsezos,M.
  29. Biotech. Bioeng. v.25 The role of chitin in uranium asdorption by R. Arrhizus Tsezos,M.
  30. Biotech. Bioeng. v.24 The mechanism of thorium biosorption by Rhizopus arrhizus Tsezos,M.;B.Volesky
  31. Biotech. Bioeng. v.24 The mechanism of thorium biosorption by Rhizopus arrhizus Tsezos,M.;B.Volesky