한국환경보건학회지 (Journal of Environmental Health Sciences)
- 제22권1호
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- Pages.51-56
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- 1996
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- 1738-4087(pISSN)
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- 2233-8616(eISSN)
ELISA법에 의한 mouse의 혈청 및 조직중의 T-2 toxin의 검색
The Detection of T-2 toxin in Serum and Organ of Mouse by ELISA
초록
In order to detect the T-2 toxin accumulation in the animal tissues, T-2 toxin, produced by Fusarium sporotrichioides M-1-1, was injected to mouse by 0, 1 and 2 mg per kilogram of body weight, respectively, and T-2 toxin extracted from serum and organs were analyzed by the indirected competitive ELISA. The indirect competitive ELISA established in the laboratory can be check less than 0.1 ppb level of T-2 toxin and average recovery of T-2 toxin spiked was 80~113% in animal samples such as serum, liver and kidney. After 6 weeks of treatment with 2 mg of T-2 toxin per kg body weight, T-2 toxin was accumulated in serum (133.0 ng/ml), liver(1.4 ng/g) and kidney(14.3 ng/g) of mouse injected with 2 mg of toxin per kg body weight.