Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Efficiency of Production of Cloned Embryos by Nuclear Transplantation with Nuclear Donor Embryos of Different Cell-stages in Rabbits

토끼에서 공핵수정란의 발달단계에 따른 복제수정란의 생산 효율

  • Published : 1996.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to evaluate the efficiency of production of cloned embryos by nuclear translatation (NT) when using 4-cell to compact morula stage embryos as nuclear donor. In micromanitulation and electrofusion of blastomeres from 4-cell to morula stage embryos, the successful injection rate was higher with late stage blastomeres, on the contrary the fusion rate was lower. The in vitro developmental rate of NT embryos was not significantly different between cell-stages of donor blastomeres. Although the overall rate of production of cloned embryos with 4-cell. 8-cell, early and late morula stage embryos was 14.0, 18.0, 15.3 and 14.1%, respectively, the mean number of blastocysts produced with a donor embryo was the most (4.51) with the compact morulae. Therefore, it can be suggested that the embryos at thelate stage is more beneficial for the mulciple production of cloned embryos, If the late stage blastomeres have maintained their totipotency to produce intact offspring.

Keywords

References

  1. Theriogenology v.33 Production of identical bovine offspring by nuclear transfer Bondioli KR;Westhusin ME;Loomey CR
  2. Biol Reprod v.50 Improved development to blastocyst of ovine nuclear transfer embryos reconstructed during the presumptive S-phase of enucleated activated oocytes Campbell KHS;Loi P;Cappai P;Wilmut
  3. Biol Reprod v.43 Factors affecting the efficiency of nuclear transplantation in the rabbit embryo Collas P;Robl JM
  4. Biol Reprod v.45 Relation between nuclear remodeling and development in nuclear transplant rabbit embryos Collas P;Robl JM
  5. Biol Reprod v.46 Influence of cell stage of the donor nucleus on development of nuclear transplant rabbit embryos Collas P;Balise JJ;Robl JM
  6. Theriogenology v.35 Development of rabbit nuclear transplant embryos from morula and blastocyst stage donor nuclei Collas P;Robl JM
  7. Cell v.23 Nuclear transplantation in Musculus : Developmental potential of nuclei from preimplantation embryos Illmensee K;Hoppe PC
  8. Korean J Emb Trans v.11 no.2 Development of mouse embryos reconstituted with 4-cell nuclei at metaphase with nocodazole Kwon OY;Kono T
  9. Bioi Reprod v.50 Bovine inner cell mass cells as donor nuclei in the production of nuclear transfer embryos and calves Keefer CL;Stice SL;Matthews DL
  10. Biol Reprod v.37 Nuclear transplantation in the bovine embryo: assessment of donor nuclei and recipient oocyte Prather RS;Barnes FL;Sims MM;Robl JM;Eyestone WH;First NL
  11. Biol Reprod v.40 Influence of nuclear and cytoplasmic activity on the early development in vivo of sheep embryos after nuclear transplantation Smith LC;Wilmut I
  12. Biol Reprod v.39 Nuclear reprogramming in nuclear transplant rabbit embryos Stice SL;Robl JM
  13. Biol Reprod v.54 Pluripotent bovine embryonic cell lines direct embryonic development following nuclear transfer Stice SL;Strelchenko NS;Keefer CL;Mathews L
  14. Nature v.320 Nuclear transplantation in sheep embryos Willadsen SM
  15. Biol Reprod v.47 Nuclear totipotency of cultured rabbit morulae to support fullterm development following nuclear transplant Yang X;Jiang S;Kovacs A;Foote RH
  16. Mol Reprod Dev v.27 Potential of hypertonic medium treatment for embryos micromanipulation Ⅱ. Assesment of nuclear transplantation methodology, isolation, subzona insertion, and electrofusion of blastomeres to intact of functionally enucleated oocytes in rabbit Yang X;Zhang L;Kov cs A;Tobback C;Foote RH
  17. Theriogenology Blastosysts as donors for nulear transfer in cattle Zakhartchenko V;Palma G;Wolf E;Brem G