Journal of Food Hygiene and Safety (한국식품위생안전성학회지)
- Volume 11 Issue 4
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- Pages.287-290
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- 1996
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- 1229-1153(pISSN)
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- 2465-9223(eISSN)
Enzyme Immunoassay for the Sulfamethazine Residues in Pork Tissue
- Park, Jun-Hong (College of Agriculture, Cheju National University, Cheju 690-756) ;
- Lim, Yoon-Kyu (College of Agriculture, Cheju National University, Cheju 690-756)
- Published : 1996.12.01
Abstract
To control the maximum residue level (MRL) for sulfamethazine (SMZ) residues in pork tissue, a microbial inhibition method is a regulatory screening assay method in Korea. Microwell plate-based competitive enzyme immunoassay (ELISA) kit is avalable for routine screening of SMZ residues in pork tissue. One ELISA kit is evaluated. Phosphate buffer extracts of samples fortified with SMZ at 0, 1, 5, and 10 ng/g were used in a recovery test of the kit. Market pork samples were assayed by the kit. Recovery of sulfamethazine was 104% at 10 ng/g. Intraassay variations and interassay variations for the kit were 7.70% and 5.76%, respectively. Concentration causing 50% inhibition of color development compared with blanks was 16.4ng. The violative pork samples with over MRL (0.1