더덕(Codonopsis lanceolatae Radix) 추출물이 면역세포에 미치는 영향

Effect of Codonopsis lanceolatae Radix Water Extract on Immunocytes

더덕 물추출물이 면역세포에 미치는 영향에 대해 연구한 결과, 더덕 물추출물을 경구투여한 경우 흉선세포의 증식을 촉진하였으나, 흉선세포에 직접 처리한 경우는 그다지 영향을 주지 않았다. 경구투여시 TH 세포를 활성화하였다. 더덕물추출물은 경구투여시 복강 매크로파지의 NO 생성을 억제하였고, 사람 PMN 세포의 phagocytosis를 증가시켰다. 이 결과는 더덕이 생체내에서 면역작용을 증강시킬 수 있는 것을 시사한다.

The purpose of this research was to investigate the effect of Codonopsis lanceolatae Radix water extract(CLE) on immunocytes. The effect of CLE on the proliferation of thymocytes was estimated by MTT colorimetric assay, sub-population of thymocytes was estimated by laser flow cytometry, production of nitric oxide from macrophages was estimated by Griess method, and phagocytosis of human polymorphonuclear cells was estimated by lucigenin chemiluminescence. CLE increased the proliferation of thymocytes in vivo, but did not affect the proliferation of thymocytes in vitro. CLE accelerated the activation of heper T cells in thymocytes. CLE inhibited the production of nitric oxide from peritoneal macrophages, and increased the phagocytosis of human polymorphonuclear cells. These results suggest that CLE have immuno-regulatory action in vivo.