Journal of Plant Biology
- Volume 39 Issue 2
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- Pages.127-135
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- 1996
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- 1226-9239(pISSN)
Somatic Embryogenesis and Plant Regeneration from Immature Zygotic Embryo Culture in Pepper (Capsicum annuum L.)
- Jo, Jeong-Yon (Department of Biology, Seoul National University) ;
- Choi, Eun-Young (Department of Biology, Seoul National University) ;
- Choi, Dong-Su (Department of Biology, Seoul National University) ;
- Lee, Kwang-Woong (Department of Biology, Seoul National University)
- Published : 1996.06.01
Abstract
An efficient system of somatic embryogenesis was established for the red pepper plant (Capsicum annuum L. cv. Nokkwang) usign immature zygotic embryos. The size of the immature zygotic embryos and the concentrations of 2, 4-D and sucrose were found to be critical. Somatic embryos were induced via callus or directly from explants and regenerated into plantlets successfully. When zygotic embryos 1~2 mm long were cultured on the modified Murashige-Skoong (MS) medium supplemented with 2 mg/L 2, 4-D for 3 weeks in the dark, somatic embryos were induced directly from the apical region of zygotic embryos with the highest frequency being approximately 90%. To mature the somatic embryos, ABA and an ethylene inhibitor AgNO3 were used. The highest frequency of shoot regeneration (25% in each) resulted at 2
Keywords
- pepper(Capsicum annuum L.);
- immature zygotic embryo;
- somatic embryogenesis;
- ethylene inhibitor;
- scanning electron micrpscory