Journal of Embryo Transfer (한국수정란이식학회지)
- Volume 11 Issue 3
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- Pages.283-289
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- 1996
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
Study on Sex Determination and Detection of Male Specific DNA Band in Bovine IVF Embryos Using Polymerase Chain Reaction
PCR 기법에 의한 소 수정란의 웅성 특이적 DNA Band 출현과 성 판별에 관한 연구
Abstract
This study was carried out to determine the sex of genomic and embryonic DNA using polymerase chain reaction(PCR). Bovine specific(216bp) and Y chromosome speicific DNA primers(l4lbp) were synthesized and tested for sexing. Bovine embryos used in this study were produced by in vitro fertilization. Few blastomeres for PCR were bisected by nicromanipulator and demi -embryos were cultured in TCM 199 medium containing 0.1% of solcoseryl. The results obtained were as follows; 1. Average optical density of genomic DNA extracted from blood of Hanwoo was 1.79