한국수정란이식학회지 (Journal of Embryo Transfer)
- 제11권3호
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- Pages.241-248
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- 1996
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- 2508-755X(pISSN)
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- 2288-0178(eISSN)
체외성숙시간 및 배양방법에 따른 한우 체외수정란의 생산효율
Production Efficiency of In Vitro Fertilized Embryos by Different Maturation Periods and Culture Systems in Korean Native Cattle
Abstract
This study was conducted to improve the production efficiency of in vitro produced (IVP) embryos in Korean Native cows. The optimal conditions and procedures for in vitro maturation(IVM), in vitro fertilization(IVF) and in vitro culture(IVC) of bovine follicular oocytes and IVP embryos were evaluated. Immature follicular oocytes were collected fiom the follicles of bovine ovaries obtained from abattoirs. The oocytes of Grade I and II for IVM were cocultured with monolayered bovine oviductal epithelial cells(BOEG) or granulosa cells in TCM-199 solution supplemented with follicle stimulating hormone, lutenizing hormone, estradiol-17