Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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페놀분해 효모 Candida tropicalis PW-51의 분리 및 분해특성

  • 김성빈 (한국과학기술연구원 생명공학연구소) ;
  • 김치경 (충북대학교 미생물학과) ;
  • 김희식 (한국과학기술연구원 생명공학연구소) ;
  • 이창호 (한국과학기술연구원 생명공학연구소) ;
  • 신기선 (한국과학기술연구원 생명공학연구소) ;
  • 권기석 (한국과학기술연구원 생명공학연구소) ;
  • 윤병대 (한국과학기술연구원 생명공학연구소) ;
  • 오희목 (한국과학기술연구원 생명공학연구소)
  • Published : 1996.12.01
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Abstract

For the biological treatment of phenolic resin wastewater containing phenol and formaldehyde, a phenol-degrading yeast was isolated from the papermill sludge, and then identified as Candida tropicalis PW-51 according to morphological, physiological and biochemical properties. The strain was able to degrade high phenol concentrations up to 2,000mg/l within 58 hours in batch cultures. Phenol-degrading efficiency by the strain was maximum at the culture conditions of a final concentration of 9 $\times$ 10$^{6}$ cells/ml, 30$\circ$C and pH 7.0. The mean degradation rate of phenol was highest at 45.5mg/l/h in 1,000mg/l phenol from 500mg/l to 2,000mg/l phenol. Because the enzyme activity of catechol 1,2-dioxygenase increased in the course of degradation of phenol, it seems that this strain degrades phenol via the ortho-cleavage of benzene ring. The isolate C. tropicalis PW-51 could be effectively used for the biological treatment of phenolic resin wastewater.

Keywords

References

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