Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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고정상세포분리기의 개발 및 Cyclosporin A 생산을 위한 고정화 연속배양공정에의 적용

  • 이태호 (한국과학기술원 화학공학과 및 생물공정연구센터) ;
  • 박성관 (강원대학교 생명과학부) ;
  • 장용근 (한국과학기술원 화학공학과 및 생물공정연구센터) ;
  • 전계택 (강원대학교 생명과학부)
  • Published : 1996.12.01
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Abstract

We have developed an efficient immobilized cell separator for continuous operation of immobilized fungal cell cultures, and applied this separator to actual fermentation process for the production of cyclosporin A (CyA), a powerful immunosuppressant. In the experiments employing highly viscous polymer (carboxymethyl cellulose) solution, the decantor showed good separating performances at high solution viscosites and fast dilution rates. Air duct and cylindrical separator installed inside the decantor turned out to play key roles for the efficient separation of the immobilized cells. By installing the decantor in an immobilized perfusion reactor system (IPRS), continuous immobilized culture was stably carried out even at high dilution rate for a long period, leading to high productivities of free cells and CyA. Almost no immobilized biomass existed in effuluent stream of the IPRS, demonstrating the effectiveness of the decan- tor system for a long-term continuous fermentation. It was noteworthy that we could obtain these results despite of the unfavorable fermentation conditions, i.e., reduced density of the biosupports caused by overgrowth of cells inside the bead particles and existence of high density of suspended fungal cells (10g/l) in the fermentation broth.

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References

  1. Biotechnol. Bioeng. v.25 Confining mycelial growth to porous microbeads : A novel technique to alter morphology of non-Newtonian mycelial cultures Gbewonyo,K.;D.I.C.Wang
  2. Biotechnol. Bioeng. v.25 Enhancing gasliquid mass transfer rates in non-Newtonian fermentaions by confining mycelial growth to microbeads in a bubble column Gbewonyo,K.;D.I.C.Wang
  3. J. Biotechnol. v.9 Immobilization of Tolypocladium inflatum spores into porous celite beads for cyclosporine production Chun,G.T.;S.N.Agathos
  4. Biotechnol. Bioeng. v.37 Comparative studies of physiological and environmental effects on the production of cyelosporin A in suspended and immobilized cells of Tolypocladium inflatum Chun,G.T.;S.N.Agathos
  5. Korean J. Biotechnol. Bioeng. v.11 Studies on mass production of intracellularly-produced secondary metabolite, cyclosporin A by use of immobilized fungal cells in stirred-tank immobilized perfusion reactor system (IPRS) Chun,G.T.;T.H Lee;Y.K.Chang
  6. Immobilized Cells: Basics and Applications Bioprocess engineering considerations in cyclosporin A fermentation by immobilized fungus Tolypocladium inflatum Lee,T.H.;G.T.Chun;Y.K.Chang;R.H.Wijffels(ed.);R.M.Buitelaar(ed.);C.Bucke(ed.);J.Tramper(ed.)
  7. Chem. Eng. Commun. v.40 Influence of non-newtonian flow behavior on mass transfer in bubble columns with and without draft tubes Kawase,Y.;M.Moo-Young
  8. Biotechnol. Bioeng. v.32 External-circulation-loop airlift bioreactors: study of the liquid circulating velovity in highly viscous non-Newtonian liquids Popovi,M.;C.W.Robinson
  9. Chem. Eng. Sci. v.42 The Specific interfacial area in external-circulation-loop airlifts and a bubble colunm-Ⅱ: carboxylmethyl cellulose/sulphite solution Popovi,M.;C.W.Robinson
  10. Biotechnol. Bioeng. v.31 Hydrodynamics and oxygen transfer in pneumatic bioreactor devices Chisti,M.Y;M.Moo-Young
  11. Bioprocess Eng. v.8 Gas holdup and mass transfer characteristics of carboxymethyl cellulose solutions in a bubble column with a radial gas sparger Ryu,H.W.;Y.K.Chang;S.D.Kim
  12. Biotechnol. Bioeng. v.27 Oxygen transfer in liquids Stejskal,J.;F.Pot ek.
  13. Chem. Eng. Sci. v.42 The specific interfacial area in external-circulation-loop airlifts and a bubble column-Ⅰ: aquenous sodium sulphite solution Popovi,M.;C.W.Robinson
  14. Korean J. Biotechnol. Bioeng. v.11 Determination of optimum bead size by calculating effectiveness factors in cyclosporin A fementation by immobilized cells Chun,G.T.;T.H.Lee;Y.K.Chang
  15. Ph.D. Thesis. Dept. of Chem. & Biochem. Eng. Physiological factors in cyclosporin A production by Tolypocladium inflatum Lee,J.
  16. Eur. J. Appl. Microbiol. v.3 Cyclosporin A and C Dreyfuss,M.;E.Harri;H.Hofmann;H.Kobel.;W.Pache;H.Tscheter
  17. Eur. J. Appl. Microbiol. v.14 Directed biosynthesis of cyclosporins Kobel,H.;R.Traber
  18. Cyclosporin. Preogress in Allergy v.38 Cyclosporin and its future Borel,J.F.;J.F.Borel(ed.)
  19. Phytochem. v.23 Biosynthesis of cyclosporin A Zocher,R.;N.Madry;H.Peeters;H.Kleinkauft
  20. J. Biol. Chem. v.262 Enzymatic synthesis of cyclosporin A Billich,A.;R.Zocher
  21. J. Antibiotic v.42 Cell free synthesis of new cyclosporins Lawen,A.;R.Traber;D.Geyl;R.Zocher;H.Keinkauf
  22. J. Antibiotic v.42 Cyclosporins-new analogues by precursor directed biosynthesis Traber,R.;H.Hofmann;H.Kobel
  23. Biotechnol. Bioeng. v.25 Thienamycin production by immobilized cells of Streptomyces cattleya in a bubble column Arcuri,E.J.;J.R.Nichols;T.S.Brix;V.G.Santamaria;B.C.Buckland;S.W.Drew
  24. Biotechnol. Lett. v.5 Semicontinuous and continuous production of the antibiotic patulin by immobilized cells of Penicillium urticae Deo,Y.M.;G.M.Goucher
  25. Appl. Microbiol. Biotechnol. v.24 Continuous production of oxytetracycline by immobilized growing Streptomyces rimosus cells Ogaki,M.;K.Sonomoto;H.Nakajima;A.Tanaka
  26. Biotechnol. Bioeng. v.26 Semicontinuous and continuous production of penicillin G by Penicillium chrysogenum cells immobilized in κ-carrageenan beads Deo,Y.M.;G.M.Goucher
  27. Biotechnol. Bioeng. v.36 The large scale immobilization of Penicillium chrysogenum: Batch and continuous operation in an air-lift reactor Keshavarz,T.;R.Eglin;E.Walker;C.Bucke;G.Holt;A.T.Bull;M.D.Lilly
  28. Korean J. Biotechnol. Bioeng. v.9 Comparative bioreactor studies in terms of oxygen transfer between suspended and immobilized fungal systems for cyclosporin A fermentation Chun,G.T.
  29. Appl. Microbiol. Biotechnol. v.21 Effect of nitrogen supplementation on the longevity of antibiotic production by immobilized cells of Penicillium urticae Deo,Y.M.;G.M.Gaucher