한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제24권6호
  • /
  • Pages.712-716
  • /
  • 1996
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

재조합효모 배양에서 비이온성 계면활성제가 외래 Glucoamylase 생산 및 분비에 미치는 영향

  • 차형준 (서울대학교 화학공학과 및 유전공학연구소) ;
  • 유영제 (서울대학교 화학공학과 및 유전공학연구소)
  • 발행 : 1996.12.01
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초록

The effects of non-ionic surfactants (Triton X-100 and Tween 80) on cloned glucoamylase production and secretion in recombinant Saccharomyces cerevisiae culture were studied. Even though the extracellular glucoamylase activity was increased by addition of Tween 80 due to the increase of the cell mass, Tween 80 did not play a role in the increase of glucoamylase secretion. On the addition of Triton X-100 addition, the secretion efficiency was increased while the cell growth was inhibited. Triton X-100 was added to the culture broth after 24 hr of culture to minimize the inhibition of the cell growth, and consequently the glucoamylase activity in the culture broth was increased by 12%.

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