한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제24권6호
  • /
  • Pages.687-692
  • /
  • 1996
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Aspergillus neger SFN-416으로부터 생산한 Xylanase II의 분리정제 및 특성

  • 성찬기 (중앙대학교 화학과) ;
  • 이상원 (경상대학교 식품공학과) ;
  • 박석규 (순천대학교 식품영양학과) ;
  • 손봉수 (경상대학교 식품공학과)
  • 발행 : 1996.12.01
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초록

Xylanase (EC 3.2.1.8) was purified approximately 4.3 fold from Aspergillus niger SFN-416 by ammonium sulfate fractionation, Sephadex G-100 gel filtration and DEAE-Sephacel ion exchange chromatography. Molecular weight of the enzyme was approximately 42,000 daltons. The optimum pH and temperature of the enzyme activity were 5.5 and 50$\circ$C, respectively. The enzyme activity was enhanced by Fe$^{2+}$, and inhibited by Hg$^{2+}$. The activity was decreased by addition of methanol, ethanol, isopropanol and 1-butanol at a concentration of 10%(v/v).

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